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Importantly, based on infectivity, viral spread, and pathogenesis, all these viruses behaved like the wild type in vivo 52 and unpublished results. As controls, uninfected animals nos. Isolation of PBMCs and cell culture. Peripheral blood mononuclear cells PBMCs were isolated by Percoll gradient centrifugation as described previously Free sex dating in bradford in 47107, venous blood was collected by jugular venipuncture and mixed with EDTA, which was used as an anticoagulant 1 ml of 7. For glutathione complementation assays, cells were cultivated in serum-free AIMV a medium that does not contain glutathione Invitrogen in the presence or in the absence of mM GSH-monoethyl ester GSH-MEwhich was added to the cells before the culture medium in order to ensure an efficient effect.

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